Other

Part:BBa_K277053:Design

Designed by: James DiCarlo   Group: iGEM09_Johns_Hopkins-BAG   (2009-10-21)


3L.3_23.B3.01


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 714
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 689
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Constructed using overlap extension PCR protocol described in RFC38


Source

Synthetic Yeast

References